nth [2019-09-05 12:27:05]

apurinic/apyrimidinic endonuclease , endonuclease III, removes abasic sites during base excision repair

Locus: BSU_22340

Isoelectric point: 9.28

Molecular weight: 24.85 kDa

Protein length: 219 aa

Gene length: 660 bp

Function: DNA repair

Product: endonuclease III

Essential: no

Synonyms: jooB,ypoB

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → A/P endonucleases

Gene

Coordinates: 2,344,755 → 2,345,414

Phenotypes of a mutant

• the mutant is sensititive to exposure to H₂O₂ PubMed

The protein

Catalyzed reaction/ biological activity

• The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to UniProt)

Protein family

• Nth/MutY family (with MutY, according to UniProt)

Cofactors

• Fe-S cluster PubMed

Effectors of protein activity

• activity is stimulated by untwisting of the DNA (by DnaD, Hbs, or YonN) PubMed

Structure

• 1P59 (complex with DNA, Geobacillus stearothermophilus)

Expression and Regulation

Operons

• Genes: asnS-dnaD-nth-ypoC
  Description: PubMed

• Genes: dnaD-nth
  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• BKE22340 (Δnth::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAACACGATTGTCACCTTTT, downstream forward: _UP4_GATAAAAAAGGACTGGTGAA
• BKK22340 (Δnth::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAACACGATTGTCACCTTTT, downstream forward: _UP4_GATAAAAAAGGACTGGTGAA

References

Reviews

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Original publications

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